RiboMed Awarded patent for Methods for determining nucleic acid methylation


United States Patent 7,470,511, Hanna, December 30, 2008


Methods for determining nucleic acid methylation

Abstract

The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, and oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide anologs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.


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One response to “RiboMed Awarded patent for Methods for determining nucleic acid methylation

  1. Pingback: RiboMed has a new Home and you are invited! « Ribomed Biotechnologies, Inc.

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